mouse monoclonal anti phospho akt ser473  (Proteintech)

Journal: Arthritis Research & Therapy

Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A

doi: 10.1186/s13075-026-03763-4

Figure Lengend Snippet: Effect of circ-CCDC134 and linear-CCDC134 overexpression on the phosphorylation of AKT and STAT3. Jurkat cells were transfected with plasmid encoding circ-CCDC134, plasmid encoding linear CCDC134 and respective empty plasmid. Then the cells were further stimulated with PMA (20 ng/mL) and ionomycin (500 ng/mL) for 1 hour. A Overexpression of circ-CCDC134 increased AKT phosphorylation at Thr308. B Overexpression of circ-CCDC134 increased AKT phosphorylation at Ser473. C A representative example. D Overexpression of linear CCDC134 did not affect AKT phosphorylation at Thr308. E Overexpression of linear CCDC134 did not affect AKT phosphorylation at Ser473. F A representative example. G Overexpression of circ-CCDC134 increased STAT3 phosphorylation at Ser727. H Overexpression of circ-CCDC134 did not affect STAT3 phosphorylation at Tyr705. I A representative example. J Overexpression of linear CCDC134 did not affect STAT3 phosphorylation at Ser727. K Overexpression of linear CCDC134 did not affect STAT3 phosphorylation at Tyr705. L A representative example. (ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: The antibodies used for the Western blot analysis included rabbit monoclonal antibodies against Jun N-terminal kinases (JNK), phospho-JNK (Thr183/Tyr185), AKT, phospho-AKT (Thr308), Signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (Ser727), poly(rC)-binding protein 2 (PCBP2), the alpha isoform of protein phosphatase 2 A (PP2A) scaffold subunit A (PPP2R1A), in addition to mouse monoclonal antibodies against phospho-AKT (Ser473) and phospho-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Over Expression, Phospho-proteomics, Transfection, Plasmid Preparation